First statement: Don’t let it run dry. If you see a major leak from the bottom of the pump head or in the gap between the two pump head, please contact your local Agilent support representative. Purification Solvent Management. Open the purge valve by turning the knob on the front of the pump to the left until you feel some resistance. Solvent Degasser – removes air gases from the solvents as they flow to the HPLC pump. Yes it looks like this! Now the valve is ok and there’s no more leak, maybe now the frit is soaked by the mobile phase and fits better?! Thanks!! reply from dmarchini< dmarchini >…. Perform flow rate check. Cap seal for purge valve knob; Cleaning swabs; Figures. Purging the solvent pumps 1. Flow anomalies can interfere with the chromatography and stand in the way of repeatable results. We routinely see HPLC systems with white fluffy crystals built up around the pump heads, pistons and various fittings from lack of maintenance on a daily basis. 0 ml/min flow for more than 2. The easy to use and economically designed LC-40D pump enables reliable HPLC chromatography in isocratic, binary, or LPGE modes. . 3. Purge the pump, check general functionality (for instance with a diagnostics test), read pre-compression value from pump panel and compare against values from manual (Thermo Scientific Dionex pumps only). 2 ml/min) for a moment and than went back to 300. The user manual for the Agilent G1311A Quaternary Pump, a high-performance liquid chromatography (HPLC) module that provides reliable gradient formation and precise flow rates. It also enables quaternary solvent delivery at. You may. 1:59. The pressure does not rise. 05 min is normal however the behavior of the method should be used to determine what is a normal variation. The pump inlet port is closed to allow the pump to run with the gas ballast valve in the open position, which helps to purge the pump. For more mobile phase flexibility in any ultrahigh speed and ultrahigh resolution application, the Thermo Scientific™ Dionex™ Ultimate™ HPG-3400RS Rapid Separation Binary Pump offers more solvent capability. The 1260 Infinity II Flexible Pump combines high performance and simplified operation with highest flexibility in automated gradient formation and solvent blending. 0Mb)Page 35: Purging The Pump The pump should never be used for priming/purging empty tubings (never let the pump N O T E run dry). 2 Set the flow of the pump at 1ml/min. Run at high flow: Next, Dolan advises to run the pump for several minutes at high flow (e. Overall Routine Pump Maintenance 1. 1:55. Perform search. After purging is done click [Pump on] button / to deliver the solvent for at least 20 to 30min to rinse the flow line . HPLC Principle. Vent. I am working with HPLC Shimadzu 20AD fitted with LC20AD. If you have a pump with an automatic purge valve, read the note below the article. This is needed to purge any air from the tubing, introduce fresh mobile phase to each line and then toHPLC, and biocompatible pumps. If adequate purging cannot be achieved with auto-purging or a suction tube does not contain liquid, for example, use the following procedure to manually purge with a syringe. Some pumps will require to manually open the purge valve and in others it is done via the software. 액상미립자측정기에서의 purge는 측정완료된 샘플용액을 제거하기 위해 사용하는 것 같다. This pump delivers reproducible gradients for routine HPLC or UHPLC applications with UV or MS detection where speed, resolution, and uncompromised data quality are essential. 6. 10 Right mouse click on the pump and choose pump wash purifier. Check for leaks and perform PM. When launching a long and continuous analysis sequence, for example 40 samples (analysis of each sample. DO NOT PUMP ANY FLUSHING SOLVENT THROUGH THE SEAL WASH LINE, OR INJECTION NEEDLE LINES. Initial System inspection It is always worthwhile to spend a few minutes before starting analysis on assessing the system suitability for the analysis task in hand. This technique is commonly used in labs for the purpose of separating, identifying, and quantifying components in a mixture. Replace piston seals. Manually prime using a priming syringe: Insert the priming syringe into the Luer fitting at the center of the draw-off valve handle. The table below describes the material covered in this book. HPLC is an abbreviation for High Performance Liquid Chromatography. The historical evolution of HPLC pumps and their characteristics has been reviewed in many textbooks (1–3), as well as journal articles (4–6). Ensure pump components are seated correctly. Page 103 • Operation Figure 29: Dwell volume. Remove and clean pistons. 000, Ready, 0. Over time, these frits will accu. Santa Clara, CA 95051. Once the system is purged, set the flow rate at 0. Run at least 25 ml buffer through degasser and pump (5 minutes) e. The pressure can be monitored at your analog output signal, with the handheld controller,With the Waters Arc HPLC System, you can count on high-efficiency separations and quality data to meet your regulatory requirements with confidence. 0*Steps to follow: To backflush the needle seat according to the following procedure, you need a pump with a manual purge valve. Pump a 20% nitric acid/water solution through the pump for 3 minutes. HPLC pumps are designed to pump liquid, not air. Purge and condition lines A1, B1 using pathway 1, then A2, B2 using pathway 2 using a 50:50 composition to ensure both pumps are taking and delivering the same volume of HPLC flushing solvent. Thus, the gradient delay volume of an HPLC system is the volume that the pump must deliver until a change in solvent composition reaches the column inlet. Flush the pump head with an appropriate solvent using the purge function; Turn off the pump flow and wait until the system pressure is zero; Analysis. A = 100%). Up to 6 FRC-40’s can be configured. c. Prepare the Pump • Use fresh or different mobile phase (as required). See your pump modules user manual for detailed instructions on re-assembly. Search by lot number or partial lot number. For GPC columns, remove column from the flow path so nothing is accidently flushed into the column. This purging is carried out for several hours or overnight, depending on the system's size and the degree of contamination. Delivering Scientific Insights to Improve Human Health and Well-Being. Optional solvent selection valves can be added for solvent. During step 1, check that the solvent is freely moving through the solvent lines. Purge pump at high flow rate (e. Detector – responds to the separated analytes emerging from the HPLC. That could help to reduce air bubble generation. 8. Where can blockages occur? Where are the consumable parts? Where can leaks occur ? What can I do to eliminate, reduce or anticipate potential problems with the LC ? Filters. Removing dissolved gases from the mobile phase improves the performance andSo that is how you fix an air bubble in the pump. Use solvent inlet filters to protect the system from incoming particles. Vacuum degassing: the solvent is exposed to a vacuum and the reduced pressure removes more than 60% of the dissolved air. Overlay of 6 pump proportioning chromatograms in 10% gradient. 0 hours and also use 50% Methanol after water flushing. ID stainless steel tubing kit • Galaxie Chromatography Software-0. You may need to remove and sonicate it. Operating principle. Run through the prime purge valve first (10 mls), then. Delivering Scientific Insights to Improve Human Health and Well-Being. then you have a little bit of work to do to really prime the line as that pump is not really powerful enough to draw in liquid if it is super dry. 06% or 0. Select this procedure when you want to prime and purge any 1525 Binary Series Pump. off. Learn essential HPLC maintenance tips. The display will show messages ‘Start PURGE’ and ‘Press ENTER to confirm’. Disconnect the line leading from the column to the detector. 5:26. When purging on LC-20AD is done, press the "Purge" bottom on SIL. Solvent Degasser – removes air gases from the solvents as they flow to the HPLC pump. . 4. b Close the black valve. But we managed to stabilize it by washing the system overnight with acetonitrile. Prime/Purge the pumps until no more air bubbles leave the pump heads. solvents. Purge the mobile phase reservoir by opening the purging valve. Count on the Arc HPLC System for high-efficiency separations and quality data to help meet your regulatory requirements with confidence. It is modified from of gas chromatography, it is applicable for both Volatile as well as Non volatile compound. It is modified from of gas chromatography, it is applicable for both Volatile as well as Non volatile compound. 00 ml/min, turn the pump back ON and close the prime purge valve to allow the liquid to flow through solvent lines, through the degasser channel, through the pump. HPLC System causes of increased pressure can include obstructions in the flow path, from the pump outlet to the mobile phase waste tube after the detector outlet. After purging the pump heads, pu rge the ProFlow flow meter for about 10 minutes. A = 100%). Replace the part that causes the obstruction. Read this guide thoroughly before using the product and operate the product in accordance with the instructions in this manual. Maintaining your LC columns. 6. September 2, 2013 . Therefore knowing how to identify it and fix it is essential to developing technical mastery in HPLC. Check that appropriate mixingThe 322 HPLC Pump is a multi-solvent pump for analytical to semi-preparative high performance liquid chromatography (HPLC). Open the black purge valve on the front of the quaternary pump by turning it several times counterclockwise. When they fill with air, they lose their prime and stop pumping. 400 bar max. The degasser is a standard feature of the 1525µ Binary HPLC Pump and an optional feature of the 1525 analytical and 1525 EF Binary HPLC Pumps. Purge HPLC pumps with buffer made in step 1. HPLC에서의 purge는 불필요한 기체를 제거하기 위해, 2. With the Arc HPLC, you can easily replicate and improve the performance of. RCM (right click mouse) in the Quat Pump module i. Operable in constant flow or constant. The pump assembly includes a pump head with an active inlet valve which has an replaceable cartridge, and an outlet valve. Attach syringe to prime/purge valve. Your purge valve is a great tool for flushing and priming your HPLC pump system. Use volatile pH modifiers as one would use for LCMS mobile phases, like formic acid, acetic acid, ammonium formate, ammonium acetate, or ammonium hydroxide. High Pressure Liquid Chromatography. ), prime system if necessary. Rebuilding the Reference Valve. With Arc HPLC System, you can easily replicate and improve the performance of your existing LC methods without compromising data quality eliminating the burdens of older, less efficient LC systems. 물론 이건 내 생각이다! 여튼 purge. Column frits are a common point of clogging. Click Okay c. • Purge each channe lwtih 25–3m. (2) Dry Priming using the HPLC pump to draw the mobile phase out of the bottles, through the lines, degasser channels and to the pump head or multichannel valve. Prepare the solvent: Begin by ensuring that the solvent is filtered and degassed. Second, the dissolved gasses must diffuse through the membrane (flat film or tubing wall). 1. Low delay volume, extended pressure range up to 800 bar, analytical flow rates up to 5. Then wash them out with water and re-assemble them on the pump head. Passivation is a metal finishing process to prevent corrosion and resist rust. The pump assembly includes a pump head with an active inlet valve which has an replaceable cartridge, and an outlet valve. Disconnect your column first and fill your mobile phase bottle with IPA (isopropyl alcohol) start your pump. the purge valve, and the actual pump mechanism. ), prime system if necessary. 6. closed. Changing the Purge Valve Frit/Purge Valve for Binary Pumps Headquarters | Other sites. 17: Ready Check Results window appear s after the NC pump blocks purge is executed 2. keep the system at this flow rate for about 10 minutes to equilibrate the system without the Column. Is the pump fuse in working order?Discover faster analysis, better resolution, and more reproducible chromatography with our new flagship UHPLC pump. (It has been simplified for clarity. • Switch “ON” the HPLC Pump. Purge the Pump (whenever changing buffers) a. 6. If [Auto Purge] is not set in the method use the manual purge option by opening the drain valve and pressing the [Purge] button on the instrument. - after changing solvents. Set the flow rate to 5 ml/min d. 8. You. OBJECTIVE: To clean and flush an Alliance HPLC System or an ACQUITY UPLC system to remove contaminants. - filter non-HPLC grade solvents - filter buffer solutions - Install an in-line filter between auto-sampler and column (removes pump seal debris, ALS rotor debris, and sample particulates). Contacting Us Vanquish Pumps (VH-P10) Operating Manual Page 3 Contacting Us There are several ways to contact us: Ordering Information For ordering information or sales. Always do this one channel at a time (e. , 10 mL/min. A damping unit is connectedThe 1260 Infinity II Quaternary Pump is a gradient pump that uses low-pressure mixing for UHPLC gradient formation and solvent blending from up to four solvents. You. Press the Purge buttons A then B on the front of the workstation. When autosampler inject, HPLC pressure pump falls down and rise again. Left pump connected to Vector PC post column derivatization. Pump Valves for HPLC. ResearchGate | Find and share researchpump for extended seal lifetime † Setting upper and lower pressure limits will turn off the pump, e. 8 um columns. 2HPLC is a High Performance liquid Chromatography. And, remember, if you have a dual-piston pump, you have two piston seals to replace. 19 Section 3: Pump Maintenance 20 Purge Valve 21 Outlet Ball Valve 21 Active Inlet Valve (AIV) 22 Seals and Pistons 25 Pump Head 26 Parts and Supplies 27 Solvent Inlet FiltersChromaster HPLC 5110 pump with auto-purge valve. The dual piston design has minimal pulsation for stable baselines. Products. After the pump, the mobile phase encounters the autosampler. Figure 2 shows how to perform the purge for an i-Series. See Reference 1 for interactive video. This pump enables quaternary solvent delivery at pressures up to 1300 bar and flow rates up to 5 mL/min. A purge valve including a PTFE frit is fitted at the pump outlet for convenient priming of the pump head. Manual - isocratic HPLC System G1380AA standard configuration • G1310A isocratic pump with solvent cabinet • G1328A manual injector (MI) • G1314A variable wavelength detector (VWD) with standard flow cell (10 mm path length, 14 µl volume, 40 bar maximum pressure) • G2220AA 2D-Value Solution ChemStation other modules that can be addedFailure to use fresh clean pickup filters in your HPLC may lead to total contamination of the flow path (column, pump, A/S and detector(s)). (Prime each pump head separately. • Purge each channe lwtih 25–3m. Featured. L/mni for 5mni . Rough pumps stay on so the system remains under vacuum. You basically tell it to shut down when the pressure drops to a specified level. 02 in. 9 Purge a short while longer, and then close the purge valve. Preparation steps: Flush the pump head with an appropriate solvent using the purge function. Peaks Peak area precision Pressure Retention times Baseline Troubleshooting overview Running into issues with your HPLC or UHPLC analysis?8th May 2023 HPLC Pump Care and Troubleshooting Many of the technical support inquiries we receive at Chrom Tech are related to care and troubleshooting of our. Seal wash holes Allow you to flush and clean the plunger seals. New InletWeaver using proven Jet Weaver technology The 1290 Infinity Quaternary Pump offers the highest accuracy andAir or particulates in head of pump or valves of the pump Leakage in transmission lines Solution • Avoid formation of negative pressure, utilisation of equalising valves in the caps of the eluent flask, prepare fresh buffer • Check tightness, if necessary, replace seals or valves • Purge the pumps with water or IPA, flush waterFirmenich Dubai. For more information, visit tutorial demonstrates how to prime the pumps in Bio-Rad's new NGC liquid chromatography sy. Turn off the pump by pressing "Pump" on the LC-20AD 4. ) If system has check valve, loosen valve to allow air to escape. Excellent gradient performance resulting from the highly accurate solvent delivery by the 5110/5160 pump with Hitachi’s unique high-speed. Pump – Open the purge valve and purge every solvent channel with 5 mL/min for 5 minutes to remove bubbles in your solvent lines (Note: if the backpressure at purging is higher than 10 bar, please replace the PTFE frit in the purge valve) – Make sure that the flushing solvent is mixable with the storage solvent of your solvent channel. 5. 2 Low Pressure No/Low Pressure Reading Question Yes No 1. Vanquish Binary Pump F: Up to 1000 bar (15,000 psi) and 8 mL/min. If seal wash option. For short gradient delay times, the gradient delay volume can be reduced to less than 70 µL. Figure 1 Overview of the binary pump Purge valve Active inlet valve Mixer Outlet valve. Gradually increase system flow up to the required flow rate,. In such case clogging of the column is most likely happening due to: additive (i. I usually let the column drip into a beaker while washing it. (Most ternary pumps actually have four solvent reservoirs. You may use the. • After few seconds, the display will show, Flow 1. Waters Student Academy 2023. L/mni for 5mni . The sensor signals the control board when the vacuum is below a preset level. If it does not, check the state of the Solvent Filters and replace them. ANSWER: The purge solvent is the solvent being pushed from the pump. We have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the pump head. After waiting a specified time, it starts pumping the mobile phase at the flow rate specified in the analytical method to automatically equilibrate the. To start purging, press the "Purge" bottom on both LC-20AD and SIL-20AC. Varian Modular Analytical HPLC Systems Figure 1. This pump operates at pressures up to 600 bar and flow rates up to 10 mL/min. The system must be primed to remove air bubbles and for the pump to function properly. A key element of its user-friendly functionality is the accessibility of the system for. 1 and purge the pumps for 5 min at a flow rate of 5 ml/min. ) If system has check valve, loosen valve to allow air to escape. with a new bottle of HPLC-grade water. Secondary Pump Head Parts 216 Purge Valve 220 Cover Parts 222 Leak Parts 224 Fuses 224 Accessory Kit 225 Tools 226 HPLC System Tool Kit 228. If the preceding step does not solve the issue, remove the purge valve and start the pump. Replace the pump head if the wetted surface is damaged, resulting in internal leaks compromising pump performance or if the pressure sensor is faulty. Liquid Chromatography. 9-0. Algae Growth in HPLC Systems 54 Prevent Blocking of Solvent Filters 55 Normal Phase Applications 57 4 Optimizing Performance 59. The ultra-high pressure capacity (up to 130 MPa/1300 bar) allows the of sub-2μm particle columns for high efficiency chromatography, especially for LC. ) If system has check valve, loosen valve to allow air to escape. In fire and explosion protection engineering, an inert (ie, non-flammable) purge gas (like nitrogen, helium, argon, etc) is introduced into an enclosed system (eg, a container or process vessel) to prevent the formation of a fuming. Algae Growth in HPLC Systems 81 Prevent Blocking of Solvent Filters 82 Normal Phase Applications 83 4 Optimizing Performance 85 Using the Degasser 86. Open the manual purge valve or use the purge command, depending on the pump type. Moreover, for system protection, the pump drive is switched off when reaching a pre-defined pressure limit. , 5-10 mL/min. Fig 2. 2 shows the flow line during LC-2010 analysis. A damping unit is connected between the two piston chambers. try to locate source of particles (sample, eluents, pump mechanics, injection valve). Achieve binary-like performance in your HPLC instrument. Liquid Chromatography (LC) Troubleshooting Resources. Use only HPLC-grade solvents and water filtered through 0. , 5-10 mL/min. a Right-click the pump icon. , 5-10 mL/min. If your problem is. Open the manual purge valve or use the purge command,. 2:19. BTW: I find it useful to tip the flat bottomed solvent. Chapter/Appendix Description Chapter 1, Overview of the Waters 1500 Series HPLC Pump Describes the. HPLC Pumps. The same problem has been observed with the Shimadzu LC-2040 C3D plus / RF HPLC chain. 45 µm inert hydrophilic filter (avoid cellulose) and degas all eluents thoroughly, using sonication under vacuum. After purging the pump heads, pu rge the ProFlow flow meter for about 10 minutes. a. Search. To wash the pump: 1. For stainless steel flow paths, proceed to Step 6; For PEEK flow paths, the cleaning procedure is completed. Pump 100% isopropanol through the pump for 3 minutes. 15 mL/min. 6. 4. Turn . The HPLC pump, often called a solvent delivery system, provides a precise flow of mobile phases of a specified composition to the column. 19 Section 3: Pump Maintenance 20 Purge Valve 21 Outlet Ball Valve 21 Active Inlet Valve (AIV) 22 Seals and Pistons 25 Pump Head 26 Parts and Supplies 27 Solvent Inlet FiltersLC-40D XR Specifications. This instalment provides an overview for analytical-scale HPLC pumps, including their requirements, modern designs,. Performing the Ramp and Decay Test After a PM. More lab efficiency with your auto delivery schedule, modify and cancel it. 02 min SD (whichever is larger) of the set flow rate. Replace the O-Ring on the Purge Valve 150 Remove the Pump Head Assembly 153 Maintenance of a Pump Head Without Seal Wash Option 155HPLC troubleshooting guide Introduction During the development of a new HPLC method, there were unexpected results—all peaks, except for the. degasser ports and/or pump head multichannel valve or pump head inlet, as needed, to draw liquid through until all lines are filled. 6. If problem still persists, contact system manufacturer. When I used the pump C last week the pressure suddenly fell down from 300 PSI to 0 (with 100% methanol, 0. It also includes relevant guidance for practical handling and mainte-(1:27min) Exchanging the Purge Valve Frit or the Purge Valve (3. Unit conversion calculator for converting commonly used unitsSJ: There are two diffusion paths involved in inline degassing (ignoring vacuum side diffusion). ), prime system if necessary. If using MassLynx or Empower. Successful HPLC Operation Part of Thermo Fisher Scientific chromatography. Introduction 4 1. Some bubbles may be generated from the suction filter head dipping in the mobile phase. 0*Today, we're diving into the exciting world of lab consumables and equipment, specifically focusing on the Shimadzu HPLC pump. For additional troubleshooting help try downloading the Thermo Scientific HPLC Troubleshooting Guide. The manual covers the overview, installation, operation, and optimization of the pump, as well as troubleshooting and maintenance tips. A purge valve including a PTFE frit is fitted at the pump outlet for convenient priming of the pump head. Seal could have fluoropolymer O-ring energizer instead (black O-ring). Seat backfl ush is enabled by a second pump, an integrated high-pressure fl ush pump. • Future-proof flow-pressure footprint provides highest application flexibility. Flow anomalies can interfere with the chromatography and stand in the way of repeatable results. 1ml/min2 g. 2 1. Set the pump to 100% 'A' at 1. g. HPLC maintenance tutorial on how to remove the pump head on an 1100/1200/1260 Agilent HPLC. • Press “Menu” till purge appears on screen. HPLC pumps incorporate single or dual piston, syringe, or diaphragm pump designs. Rebuilding the Reference Valve. Insert the suction tube for the flow channel to be purged into the reservoir bottle containing the mobile phase and place the bottle on the reservoir tray. Purge Valve Assembly: 1 - Valve body, 2 - PTFE frit with groove, 3 - Seal cap. (Prime each pump head separately. Purge pump at high flow rate (e. In other words is the system configuration comprising of the pump, injector/autosampler, column, and detector is appropriate for carrying out the analysis in. g. Open the manual purge valve or use the purge command, depending on the pump type. Wash out the blockage. The preparative LC system performs automatic simulations using the pre-preparative results. The dual piston design has minimal pulsation for stable baselines. And, remember, if you have a dual-piston pump, you have two piston seals to replace. Installing the Pump Purging the Quaternary Pump Ensure that the outlet tube is connected from the purge valve to the waste container. Dolan advises that this will clear. 7. The 1290 Infinity II Flexible Pump combines highest performance, methods compatibility, and. Use 2 um frit for 3. 3 Crucial Points to achieve ultimate quaternary pump performance (low pressure mixing!): 1. Remove sample vials from the auto-sampler tray g. Using an autosampler reduces manual labor and improves injection precision and accuracy compared to manual sample injection, an absolute priority for a quantitative HPLC analysis. Purging the solvent pumps 1. Purge the pumps each time fresh eluent or water is prepared. Replace piston seals. Purge the system to remove air bubbles and make sure the solvent is the same solvent when the GPC column is placed. 02-0. Reducing the diffusion path to improve degassing. Turn the handle counterclockwise, about 1/2-turn, to open the valve, and pull back on the plunger to remove air bubbles. Currently loaded videos. *Run to run RT variation of ±0. Each channel comprises a pump assembly including pump drive, pump head, active inlet valve with replaceable cartridge, and outlet valve. Maintaining Your Agilent 1100 Series HPLC System Optimize performance and maximize efficiency in your lab. 9. It is Liquid Chromatography. the user. Replacing the Plunger Seal and Plunger. The impact of this type of clogging will be gradual, not sudden. Power off the pump and remove the waste tubing from the purge valve. HPLC system. the user. 3. 3601-EN-5. • Vacuum sensor – A sensor on the control board that monitors the vacuum in the system. This guide describes the basic operation and troubleshooting for the ExionLCTM AD system . Flushing and purging all solvent lines and fluidics is the first step that should be done if unstable system pressure. The following diagrams illustrate the operating principle of a modern HPLC pump: an electric motor drives a piston (generally made of sapphire or ceramic material) in and out of metal, ceramic, or PEEK piston chamber. Purge valve: Reciprocating piston pumps do not work if there is air in the lines, which can be the case after changing a solvent reservoir, or leaving the pump off. See your pump modules user manual for detailed instructions on re-assembly. Page 36 6 Observe the pressure. Priming and Purging the 1525 Binary HPLC Pump. Press “Prime” button (or pump at max flow), continue to draw on syringe until no bubbles are seen. Remove and clean pistons. Select this procedure when you want to prime and purge any 1525 Binary Series Pump. Change the gradient. From the data analysis program vent the system. 액상미립자측정기에서의 purge는 측정완료된 샘플용액을 제거하기 위해 사용하는 것 같다. This pump enables quaternary solvent delivery at pressures up to 800. you will need to • General cleaning Daily first re-prime the pump by purging the air from all the lines. Purging has only helped to reduce. 1 Introduction Purpose of the User Manual The User Manual provides you with instructions and information to run the ÄKTA™ avant system. 5 um columns, use 0. We have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the pump head. Flow Connections of the Binary Pump with Solvent Selection Valve 24 Flow Connections of the Binary Pump without Solvent Selection Valve 27 Priming and Purging the System 30 2 Optimizing Performance How to optimize the binary pump to achieve best chromato-graphic results 33 Hints for Successful Use of the Binary Pump 34 Solvent. g. Open the purge valve on the Agilent Infinity 1260 isocratic pump b. • Syringe, loop & injector plunger seal. g. PENDAHULUAN Penelitia n yang berkaitan dengan tumbuhan berguna Indonesia. isolating bioactive compounds the so called High Performance Liquid Chromatography (HPLC).